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110 Characteristics and management of generalized pustular psoriasis (GPP): experience from the Central and Eastern Europe (CEE) GPP Expert Network by P. Wolf; R. Ceovic; C. Conrad; M. Marovt; V. Mateeva; J. Maul; A. Nicolescu; L. Pavlovsky; A. Szegedi; A. Reich is a Medicine article available to read on EtoBox.
What is 110 Characteristics and management of generalized pustular psoriasis (GPP): experience from the Central and Eastern Europe (CEE) GPP Expert Network about?
is a novel, monoclonal antibody that selectively targets interleukin (IL)-13 and prevents formation of the IL-13 receptor alpha 1 (Ra1)/IL-4 receptor alpha (Ra) heterodimer receptor signaling complex. A previous crystal structure report showed that lebri does not interfere with binding of IL-13 to the IL-13 Receptor alpha 2 (Ra2) decoy receptor. In contrast, other IL-13 antibodies tralokinumab and cendakimab had been reported to inhibit IL-13 binding to both IL-13Ra1 and IL-13Ra2. We wanted to investigate whether lebri binding to IL-13 interfered with IL-13 binding to IL-13Ra2 and the subsequent internalization. Through competitive binding experiments using surface plasmon resonance, we confirmed that lebri can bind to the tralokinumab/IL-13 and cendakimab/IL-13 complexes. These data showed that lebri binds to IL-13 at a different epitope. First, we induced IL-13Ra2 expression on HaCaT cells, a spontaneously transformed keratinocyte cell line from adult human skin, using co-treatment of IL-13 and TNF-alpha. Then, we fluorescently stained these cells and confirmed the increased expression of IL-13Ra2. Using live-cell confocal imaging, we observed that IL-13 can bind IL-13Ra2 and is
Who reads 110 Characteristics and management of generalized pustular psoriasis (GPP): experience from the Central and Eastern Europe (CEE) GPP Expert Network?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- P. Wolf; R. Ceovic; C. Conrad; M. Marovt; V. Mateeva; J. Maul; A. Nicolescu; L. Pavlovsky; A. Szegedi; A. Reich
- Publisher
- Elsevier BV
- Published
- 2022
- Language
- EN
- Field
- Medicine (Health Sciences)