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p38 MAPK and COX2 inhibition modulate human chondrocyte response to TGF‐β by Rebecca K. Studer; Constance R. Chu is a Medicine article available to read on EtoBox.

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## Abstract These studies compare actions of p38 MAPK inhibition and COX2 inhibition to modulate human arthritic chondrocyte responses to TGF‐β and FCS under basal and IL‐1 activated conditions. Chondrocytes isolated from arthritic human femoral condyle cartilage obtained at total knee replacement were grown to 80% confluence. Proteoglycan synthesis and proliferation were measured with and without IL‐1 activation in the presence and absence of growth factors and with and without inhibition of p38 MAPK or COX2 activity. Experiments to evaluate TIMP‐1 production under these conditions were done using cartilage organ cultures. Neither p38 MAPK inhibitors nor COX2 inhibition affected basal proliferation. However both inhibitors enhanced the proliferative response to TGF‐β and FCS in IL‐1 activated chondrocytes. TGF‐β stimulated proteoglycan synthesis was decreased by p38 MAPK inhibition, however COX2 inhibition restored the response to TGF‐β in IL‐1 activated cells. In contrast, COX2 inhibition did not modulate TIMP‐1 production while p38 MAPK inhibitors potentiated TGF‐β stimulated production of TIMP‐1 in IL‐1 activated cartilage. p38 MAPK inhibition and COX2 inhibition have unique an

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Author

Rebecca K. Studer; Constance R. Chu

Publisher

Elsevier Science; Wiley (John Wiley & Sons); Wiley-Blackwell; Wiley (ISSN 0736-0266)

Published

2005

Language

EN

Field

Medicine (Health Sciences)