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The role of different cytochrome P450 isoforms in in vitro chloroform metabolism by Emanuela Testai; Valentina De Curtis; Simonetta Gemma; Laura Fabrizi; PierGiovanni Gervasi; Luciano Vittozzi is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is The role of different cytochrome P450 isoforms in in vitro chloroform metabolism about?

The two CHCl3 activation pathways have been studied in incubations at different oxygenation conditions with hepatic microsomes from control Sprague Dawley (SD) rats or SD rats treated with different cytochrome P450 inducers (acetone, phenobarbital, pyrazole, dexamethasone, and beta-naphthoflavone). The present results provide direct evidence that CHCl3 concentration is critical in determining the role of different cytochrome P450 isoforms (CYP) and the related effects of metabolic inducers. At 0.1 mM CHCl3 concentration, the only major contribution to its oxidative biotransformation in liver microsomes from untreated rats was due to CYP2E1, as shown by metabolic inhibition due to 4-methylpyrazole or by anti-CYP2E1 antibodies. Moreover, animal treatments with acetone and pyrazole increased the production of adducts of phosgene to microsomal phospholipid by about 10-15 times. At 5 mM chloroform, in control rat liver microsomes, CYP2B1/2 was the major participant responsible for chloroform activation, while CYP2E1 and CYP2C11 were also significantly involved. Consistently, at this chloroform concentration, the effect of phenobarbital (CYP2B1/2 inducer) was maximal, producing very high

Who reads The role of different cytochrome P450 isoforms in in vitro chloroform metabolism?

Author

Emanuela Testai; Valentina De Curtis; Simonetta Gemma; Laura Fabrizi; PierGiovanni Gervasi; Luciano Vittozzi

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); VCH Publishers; Wiley (ISSN 0887-2082)

Published

1995

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)