Can I read Direct Identification of Amino Acid-derived Hormones with Single-atom Resolution Using an Engineered Aerolysin Nanopore on EtoBox?

Direct Identification of Amino Acid-derived Hormones with Single-atom Resolution Using an Engineered Aerolysin Nanopore by Jie Jiang; Junge Li; Yan Gao; Meng-Yin Li; Yi-Tao Long is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Direct Identification of Amino Acid-derived Hormones with Single-atom Resolution Using an Engineered Aerolysin Nanopore about?

nanopores, we have investigated the voltage dependent stability of mononucleosomes as well as the 12-mer nucleosome arrays. In experiments with our in vitro assembled mononucleosomes, we show that the octasomal nucleosomes, comprising of octameric histone complexes on DNA, systematically breakdown into its partial structures, which we reason to be hexasomes and tetrasomes, in a voltage dependent manner. We attribute these structural changes to the opposing electrical forces experienced by the positively charged histone protein complex and the negatively charged DNA. We next investigate the stability of 12-mer nucleosome arrays and find discrete multi-level events corresponding to discrete structures on the array molecules. A detailed analysis of these discrete levels revealed that these structures correspond to single, multiple as well as partial nucleosomes on the array molecule. We further show that these structures disassemble in a voltage dependent manner. Our work demonstrates, for the first time, application of the glass nanopore platform to perform stability studies on nucleosome-DNA complexes, both individually as well as on nucleosomal arrays. This opens up future studies

Who reads Direct Identification of Amino Acid-derived Hormones with Single-atom Resolution Using an Engineered Aerolysin Nanopore?

Author

Jie Jiang; Junge Li; Yan Gao; Meng-Yin Li; Yi-Tao Long

Publisher

Elsevier BV

Published

2023

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)