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P53 Mutations in Primary Human Lung Tumors and Their Metastases by Martin B. Reichel; Hiroko Ohgaki; Iver Petersen; Paul Kleihues is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract In a total of 26 primary human lung tumors and 60 metastases derived from them, exons 5–8 of the __p53__ tumor suppressor gene were analyzed by single‐strand conformation polymorphism and subsequent direct DNA sequencing of amplified DNA. Mutational inactivation of the __p53__ gene was identified in four of five squamous cell carcinomas, three of nine adenocarcinomas, and two of nine small‐cell carcinomas, the overall incidence being 35%. Point mutations occurred at a similar incidence in exons 5–8, with a preference for G←T transversions. In seven of nine cases (78%), mutations were identical in the primary tumor and all of its metastases, indicating that in lung tumors, __p53__ mutations usually precede metastasis and that hematogenic and lymphogenic dissemination of tumor cells to other tissues is not associated with a selection against __p53__ inactivation. In one case, a kidney metastasis had the same mutation as the primary squamous cell carcinoma, whereas a liver metastasis had no mutation, indicating heterogeneity of the primary lung neoplasm and selective metastasis of mutated and nonmutated tumor cells to kidney and liver, respectively. Only in one liver metas

Who reads P53 Mutations in Primary Human Lung Tumors and Their Metastases?

Author

Martin B. Reichel; Hiroko Ohgaki; Iver Petersen; Paul Kleihues

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0899-1987)

Published

1994

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)