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Significant variations in nuclear structure occur between and within Gleason grading patterns 3, 4, and 5 determined by digital image analysis by Robert W. Veltri; Cameron Marlow; Masood A. Khan; Michael C. Miller; Jonathan I. Epstein; Alan W. Partin is a Medicine article available to read on EtoBox.

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## Abstract ## BACKGROUND Alterations in nuclei structure and DNA content captured from Gleason grading patterns 3, 4 and 5 of radical prostatectomy (RP) cases were determined by a computer‐assisted microscope. Quantitative Nuclear Morphometry (QNM) profiles were created to evaluate variability in nuclear structure within each of these grades. ## METHODS A tissue microarray (TMA) was constructed using RP cases and the prostate cancer (PCa) TMA cores prepared from 20 GG‐3, 9 GG‐4, 10 GG‐5 patterns, and 20 benign cancer‐adjacent cases from RP archival paraffin blocks. Feulgen‐stained nuclei were captured from 0.6 mm spots using the AutoCyteTM system. Pools of 1100 nuclei captured from each test group were used to calculate Multivariate Logistic Regression (MLR) models that generated predictive indices and predictive probabilities (PP) to make comparisons between and within each set of pooled nuclei. ## RESULTS A single QNM profiles yielded areas of receiver operator characteristic curves (ROC) that distinguished differences among benign cancer‐adjacent nuclei and GG‐3 (ROC‐AUC = 0.78); GG‐4 (ROC‐AUC = 0.86) and GG‐5 (ROC‐AUC = 0.88) with accuracies of 73%, 78% and 80% respectively. A

Who reads Significant variations in nuclear structure occur between and within Gleason grading patterns 3, 4, and 5 determined by digital image analysis?

Author

Robert W. Veltri; Cameron Marlow; Masood A. Khan; Michael C. Miller; Jonathan I. Epstein; Alan W. Partin

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0270-4137)

Published

2007

Language

EN

Field

Medicine (Health Sciences)