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Can I read Amyloidogenic and Non-amyloidogenic Molten Globule Conformation of β-Lactoglobulin in Self-crowded Regime on EtoBox?
Amyloidogenic and Non-amyloidogenic Molten Globule Conformation of β-Lactoglobulin in Self-crowded Regime by Sara Venturi; Barbara Rossi; Mariagrazia Tortora; Renato Torre; Andrea Lapini; Paolo Foggi; Marco Paolantoni; Sara Catalini is a Materials Science article available to read on EtoBox.
What is Amyloidogenic and Non-amyloidogenic Molten Globule Conformation of β-Lactoglobulin in Self-crowded Regime about?
Molecular insights on the β-lactoglobulin thermal unfolding and aggregation are derived from FTIR and UV Resonance Raman (UVRR) investigations. We propose an in situ and in real-time approach that thanks to the identification of specific spectroscopic markers can distinguish the two different unfolding pathways pursued by β-lactoglobulin during the conformational transition from the folded to the molten globule state, as triggered by the pH conditions. For both the investigated pH values (1.4 and 7.5) the greatest conformational variation of β-lactoglobulin occurs at 80 °C and a high degree of structural reversibility after cooling is observed. In acidic condition β-lactoglobulin exposes to the solvent its hydrophobic moieties in a much higher extent than in neutral solution, resulting on a highly open conformation. Moving from the diluted to the self-crowded regime, the solution pH and consequently the different molten globule conformation select the amyloid or non-amyloid aggregation pathway. At acidic condition the amyloid aggregates form during the heating cycle leading to the formation of transparent hydrogel. On the contrary, in neutral condition the amyloid aggregates never
Who reads Amyloidogenic and Non-amyloidogenic Molten Globule Conformation of β-Lactoglobulin in Self-crowded Regime?
It is typically read by researchers, students, and practitioners in Materials Science.
- Author
- Sara Venturi; Barbara Rossi; Mariagrazia Tortora; Renato Torre; Andrea Lapini; Paolo Foggi; Marco Paolantoni; Sara Catalini
- Publisher
- Elsevier BV
- Published
- 2023
- Language
- EN
- Field
- Materials Science (Physical Sciences)