About this document
BL-21 Competent Cell Transformation Protocol by Anantathep Sanitsom is a document available to read on EtoBox.
This document provides instructions for transforming BL-21 cells through a 12 step procedure: BL-21 competent cells are thawed and mixed with plasmid DNA, heat shocked, recovered on ice, grown in LB media, pelleted, resuspended in residual media, and plated on LB-Amp plates before incubating overnight to select transformed colonies for further use. Key steps include heat shocking the cells at 42°C for 90 seconds to facilitate DNA uptake, recovering in media for protein expression, and selecting singular col
- Author
- Anantathep Sanitsom
- Language
- EN