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Transgenic cattle are produced through three main methods: DNA microinjection, retrovirus-mediated gene transfer, and embryonic stem cell-mediated gene transfer. DNA microinjection involves transferring a desired gene construct into the pronucleus of a reproductive cell, which is then transferred to a recipient female. Retrovirus-mediated gene transfer uses retroviruses as vectors to transfer genetic material, resulting in chimeric animals. Embryonic stem cell-mediated gene transfer isolates stem cells from

Author

Sunitha Machiraju

Language

EN