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Can I read Selective Down-regulation of Human Papillomavirus Transcription by 2-Deoxyglucose on EtoBox?
Selective Down-regulation of Human Papillomavirus Transcription by 2-Deoxyglucose by Toshiyuki Maehama; Andrea Patzelt; Maike Lengert; Karl-Josef Hutter; Koji Kanazawa; Harald Zur Hausen; Frank Rösl is a Medicine article available to read on EtoBox.
What is Selective Down-regulation of Human Papillomavirus Transcription by 2-Deoxyglucose about?
The glycolytic pathway inhibitor 2-deoxyglucose (2-DG) is capable of suppressing the transcription of the human pathogenic papillomavirus type 18 (HPV 18) in cervical carcinoma cells and derived non-tumorigenic somatic cell hybrids at the level of transcription initiation. HPV down-regulation is selective, since other reference genes are not affected or even up-regulated under the same experimental conditions. Moreover, 2-DG appears to restore the normal half-life of the tumor suppressor gene product p53, because the protein is strongly up-regulated after HPV18 E6/E7 suppression. The observed 2-DG-effect is not cytotoxic and is reversible after refeeding with fresh medium. HPV18 suppression by 2-DG can be completely abrogated by simultaneous treatment with the intracellular Ca 2؉ antagonist TMB-8, indicating that Ca 2؉ , a known intracellular ''second messenger'', is involved in this process. Elevated c-myc and p53 expression appears to be responsible for the time-dependent accumulation of apoptotic cells after prolonged 2-DG treatment. The finding that 2-DG acts selectively against the expression of a human pathogenic papillomavirus strongly suggests that an appropriate level of g
Who reads Selective Down-regulation of Human Papillomavirus Transcription by 2-Deoxyglucose?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Toshiyuki Maehama; Andrea Patzelt; Maike Lengert; Karl-Josef Hutter; Koji Kanazawa; Harald Zur Hausen; Frank Rösl
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)
- Published
- 1998
- Language
- EN
- Field
- Medicine (Health Sciences)