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What is HistoDenz 14.1% Lymphocyte Isolation Guide about?
This document provides instructions for using a 14.1% HistoDenz solution to isolate lymphocytes from blood. The solution has a density of 1.078 ± 0.002 g/ml and osmolality of 300 ± 10 mOsm. To isolate lymphocytes, blood is layered over the HistoDenz solution and centrifuged, forming a white lymphocyte layer at the interface that can be removed with a pipette. Lymphocytes may then be concentrated by dilution and further centrifugation.
- Author
- Ardizenatha
- Language
- EN