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mrtl—A translation/localization regulatory protein encoded within the human c-myc locus and distributed throughout the endoplasmic and nucleoplasmic reticular network by Hyoungsoo Choi; Nateka L. Jackson; Denise R. Shaw; Peter D. Emanuel; Y. Lucy Liu; Albert Tousson; Zheng Meng; Scott W. Blume is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is mrtl—A translation/localization regulatory protein encoded within the human c-myc locus and distributed throughout the endoplasmic and nucleoplasmic reticular network about?

## Abstract mrtl (__myc__‐__r__elated __t__ranslation/__l__ocalization regulatory factor) is a previously uncharacterized protein synthesized from the first open reading frame contained within the human c‐__myc__ P0 transcript, ∼800 nucleotides upstream of the Myc coding sequence. The mrtl protein, 114 amino acids in length, is projected to contain an N‐terminal transmembrane domain and a highly charged C‐terminal interaction domain with homology to numerous RNA‐binding proteins. Using monoclonal antibodies raised against the hydrophilic C‐terminal domain, endogenous mrtl was visualized in human breast tumor cell lines and primary mammary epithelial cells at the nuclear envelope and contiguous endoplasmic/nucleoplasmic reticulum. mrtl colocalizes and coimmunoprecipitates with translation initiation factor eIF2α and the 40S ribosomal protein RACK1, and appears capable of binding specifically to the c‐__myc__ RNA. Inducible ectopic overexpression of wild‐type mrtl interferes with the function of endogenous mrtl, which results in loss of Myc from the nucleus. Furthermore, treatment of cells with a peptide derived from the C‐terminal domain displaces endogenous mrtl and causes a dramat

Who reads mrtl—A translation/localization regulatory protein encoded within the human c-myc locus and distributed throughout the endoplasmic and nucleoplasmic reticular network?

Author

Hyoungsoo Choi; Nateka L. Jackson; Denise R. Shaw; Peter D. Emanuel; Y. Lucy Liu; Albert Tousson; Zheng Meng; Scott W. Blume

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)

Published

2008

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)