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What is Calcofluor Staining of Fungal Hyphae about?

This document describes a method for staining fungal hyphae and propagules with fluorescent brightener to allow direct visualization under an epifluorescent microscope. The method involves preparing a sample dilution, staining it with fluorescent brightener 28 at a concentration of 0.1 mg/ml, filtering it, mounting it on a slide, and observing it under an epifluorescent microscope. Calculations are provided to determine propagule number, total hyphal length, and biovolumes from the microscopy observations.

Author

rck46

Language

EN