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Happyase Enzyme Kinetics and Inhibition by anon_900161280 is a document available to read on EtoBox.
1. The researchers studied an enzyme called happyase that catalyzes the reaction of SAD to HAPPY. They found the kcat of happyase is 600 s-1. 2. In an experiment with [Et] = 20 nM, [SAD] = 40 μM, and reaction velocity V0 = 9.6 μM s-1, the Km for SAD was calculated to be 10 μM. 3. In a separate experiment with [Et] = 10 nM and V0 = 3 μM s-1, the [SAD] was determined to be the same as the previously calculated Km, which is 10 μM.
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- anon_900161280
- Language
- EN