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Localization of GTP cyclohydrolase in monoaminergic but not nitric oxide-producing cells by Onyou Hwang; Harriet Baker; Steven Gross; Tong H. Joh is a Neuroscience article available to read on EtoBox.

What is Localization of GTP cyclohydrolase in monoaminergic but not nitric oxide-producing cells about?

The first and rate-limiting enzyme in tetrahydrobiopterin (BH4) biosynthesis is GTP cyclohydrolase (GTPCH). BH4 serves as the essential cofactor for aromatic L-amino acid hydroxylases, such as tyrosine hydroxylase (TH) and tryptophan hydroxylase (TPH), as well as for nitric oxide synthase (NOS). We hypothesized that to provide access to the cofactor, a close association exists between BH4-synthesizing and BH4dependent enzymes, and we determined the relationship among GTPCH, neuronal NOS (nNOS), and TH in rat brain and adrenal gland using immunohistochemistry and in situ hybridization. Analyses of adjacent sections revealed specific localization of GTPCH in TH-containing cells of the substantia nigra, ventral tegmental area, hypothalamus, locus ceruleus, and adrenal medulla, and also in TPH-containing cells of the dorsal raphe nucleus and pineal gland. Thus, BH4 can be synthesized in all monoaminergic cells and is readily available for the enzymes requiring it. In contrast, analysis of adjacent sections showed that nNOS was not colocalized with GTPCH. Scattered nNOS-positive cells were found in the cortex, striatum, cerebellum, and olfactory bulb, all areas that receive monoaminergi

Who reads Localization of GTP cyclohydrolase in monoaminergic but not nitric oxide-producing cells?

Author

Onyou Hwang; Harriet Baker; Steven Gross; Tong H. Joh

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0887-4476)

Published

1998

Language

EN

Field

Neuroscience (Life Sciences)