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Can I read Self-assembly and Regulation of Protein Cages from Pre-organised Coiled-coil Modules on EtoBox?
Self-assembly and Regulation of Protein Cages from Pre-organised Coiled-coil Modules by Lapenta, Fabio (author);Aupič, Jana (author);Vezzoli, Marco (author);Strmšek, Žiga (author);Da Vela, Stefano (author);Svergun, Dmitri I. (author);Carazo, José María (author);Melero, Roberto (author);Jerala, Roman (author) is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Self-assembly and Regulation of Protein Cages from Pre-organised Coiled-coil Modules about?
## Abstract Coiled-coil protein origami (CCPO) is a modular strategy for the de novo design of polypeptide nanostructures. CCPO folds are defined by the sequential order of concatenated orthogonal coiled-coil (CC) dimer-forming peptides, where a single-chain protein is programmed to fold into a polyhedral cage. Self-assembly of CC-based nanostructures from several chains, similarly as in DNA nanotechnology, could facilitate the design of more complex assemblies and the introduction of functionalities. Here, we show the design of a de novo triangular bipyramid fold comprising 18 CC-forming segments and define the strategy for the two-chain self-assembly of the bipyramidal cage from asymmetric and pseudo-symmetric pre-organised structural modules. In addition, by introducing a protease cleavage site and masking the interfacial CC-forming segments in the two-chain bipyramidal cage, we devise a proteolysis-mediated conformational switch. This strategy could be extended to other modular protein folds, facilitating the construction of dynamic multi-chain CC-based complexes.
Who reads Self-assembly and Regulation of Protein Cages from Pre-organised Coiled-coil Modules?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Lapenta, Fabio (author);Aupič, Jana (author);Vezzoli, Marco (author);Strmšek, Žiga (author);Da Vela, Stefano (author);Svergun, Dmitri I. (author);Carazo, José María (author);Melero, Roberto (author);Jerala, Roman (author)
- Publisher
- Springer Science and Business Media LLC
- Published
- 2021
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)