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Quantitative Determination of Perfluorooctanoic Acid in Serum and Plasma by Liquid Chromatography Tandem Mass Spectrometry by John M. Flaherty; Paul D. Connolly; Emily R. Decker; S. Mark Kennedy; Mark E. Ellefson; William K. Reagen; Bogdan Szostek is a Chemistry article available to read on EtoBox.

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A selective and sensitive method for analysis of perfluorooctanoic acid (PFOA) in human serum and plasma, utilizing liquid chromatography tandem mass spectrometry (LC-MS/MS), has been developed and thoroughly validated to satisfy strict FDA guidelines for bioanalytical methods. A simple, automated sample preparation procedure, involving extraction of the target analyte with acetonitrile on protein precipitation media in a 96-well plate format was developed, allowing efficient handling of large numbers of samples. The proposed method uses the calibration standards prepared in a surrogate matrix (rabbit serum or plasma) and 13 C-labeled PFOA as the internal standard to account for matrix effects, instrument drift, and extraction efficiency. Human serum and plasma could not be used for matrix matching of calibration standards as endogenous levels of PFOA observed in the control human serum and plasma significantly exceeded the targeted lower limit of quantitation (LLOQ) of the method. Precision and accuracy of the method were demonstrated by analysis of rabbit serum and plasma control samples fortified at 0.5, 5, and 40 ng/mL PFOA and human serum and plasma fortified at 1.0, 5.0, 40 n

Who reads Quantitative Determination of Perfluorooctanoic Acid in Serum and Plasma by Liquid Chromatography Tandem Mass Spectrometry?

Author

John M. Flaherty; Paul D. Connolly; Emily R. Decker; S. Mark Kennedy; Mark E. Ellefson; William K. Reagen; Bogdan Szostek

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-376X)

Published

2005

Language

EN

Field

Chemistry (Physical Sciences)