Can I read Bovine serum albumin and lysophosphatidic acid stimulate calcium mobilization and reversal of cAMP-induced stellation in rat spinal cord astrocytes on EtoBox?

Bovine serum albumin and lysophosphatidic acid stimulate calcium mobilization and reversal of cAMP-induced stellation in rat spinal cord astrocytes by Timothy J. Manning JR.; Harald Sontheimer is a Neuroscience article available to read on EtoBox.

What is Bovine serum albumin and lysophosphatidic acid stimulate calcium mobilization and reversal of cAMP-induced stellation in rat spinal cord astrocytes about?

We report that lysophosphatidic acid (LPA) stimulates dynamic calcium (Ca 21 ) fluctuations and morphological rearrangements in astrocytes derived from neonatal rat spinal cord. Addition of 10 μM LPA elicited single Ca 21 transients, or biphasic oscillations and sustained increases in intracellular Ca 21 ([Ca 21 ] i ). The biphasic Ca 21 response consisted of an initial release from intracellular stores, often followed by a sustained elevation or continued oscillations that required Ca 21 flux across the cell membrane. The type of Ca 21 response, but not the overall magnitude, was dependent on LPA concentration. Higher concentrations (.10 μM) often elicited sustained increases in [Ca 21 ] i , while lower concentrations stimulated oscillations or single Ca 21 transients. It has previously been established that agents that elevate cyclic adenosine monophosphate (cAMP) induce flat astrocytes to adopt a more stellate morphology. LPA can completely reverse this morphological change at a half-maximal concentration of 215 nM. Inhibiting LPA-induced [Ca 21 ] i fluctuations using BAPTA-AM to buffer [Ca 21 ] i and EGTA in the bath to prevent transmembrane flux had little effect on the abilit

Who reads Bovine serum albumin and lysophosphatidic acid stimulate calcium mobilization and reversal of cAMP-induced stellation in rat spinal cord astrocytes?

Author

Timothy J. Manning JR.; Harald Sontheimer

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0894-1491)

Published

1997

Language

EN

Field

Neuroscience (Life Sciences)