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Can I read Rapid quantification and taxonomic classification of environmental DNA from both prokaryotic and eukaryotic origins using a microarray on EtoBox?
Rapid quantification and taxonomic classification of environmental DNA from both prokaryotic and eukaryotic origins using a microarray by Todd Z. DeSantis; Carol E. Stone; Sonya R. Murray; Jordan P. Moberg; Gary L. Andersen is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Rapid quantification and taxonomic classification of environmental DNA from both prokaryotic and eukaryotic origins using a microarray about?
A microarray has been designed using 62,358 probes matched to both prokaryotic and eukaryotic small-subunit ribosomal RNA genes. The array categorized environmental DNA to specific phylogenetic clusters in under 9 h. To a background of DNA generated from natural outdoor aerosols, known quantities of rRNA gene copies from distinct organisms were added producing corresponding hybridization intensity scores that correlated well with their concentrations (r = 0.917). Reproducible differences in microbial community composition were observed by altering the genomic DNA extraction method. Notably, gentle extractions produced peak intensities for Mycoplasmatales and Burkholderiales, whereas a vigorous disruption produced peak intensities for Vibrionales, Clostridiales, and Bacillales.
Who reads Rapid quantification and taxonomic classification of environmental DNA from both prokaryotic and eukaryotic origins using a microarray?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Todd Z. DeSantis; Carol E. Stone; Sonya R. Murray; Jordan P. Moberg; Gary L. Andersen
- Publisher
- John Wiley and Sons; Oxford University Press; Oxford University Press (OUP); Elsevier BV (ISSN 0378-1097)
- Published
- 2005
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)