Can I read Interactions with nanoscale topography: Adhesion quantification and signal transduction in cells of osteogenic and multipotent lineage on EtoBox?

Interactions with nanoscale topography: Adhesion quantification and signal transduction in cells of osteogenic and multipotent lineage by Manus J. P. Biggs; R. Geoff Richards; Nikolaj Gadegaard; Rebecca J. McMurray; Stanley Affrossman; Chris D. W. Wilkinson; Richard O. C. Oreffo; Mathew J. Dalby is a Engineering article available to read on EtoBox.

What is Interactions with nanoscale topography: Adhesion quantification and signal transduction in cells of osteogenic and multipotent lineage about?

## Abstract Polymeric medical devices widely used in orthopedic surgery play key roles in fracture fixation and orthopedic implant design. Topographical modification and surface micro‐roughness of these devices regulate cellular adhesion, a process fundamental in the initiation of osteoinduction and osteogenesis. Advances in fabrication techniques have evolved the field of surface modification; in particular, nanotechnology has allowed the development of nanoscale substrates for the investigation into cell‐nanofeature interactions. In this study human osteoblasts (HOBs) were cultured on ordered nanoscale pits and random nano “craters” and “islands”. Adhesion subtypes were quantified by immunofluorescent microscopy and cell‐substrate interactions investigated via immuno‐scanning electron microscopy. To investigate the effects of these substrates on cellular function 1.7 k microarray analysis was used to establish gene profiles of enriched STRO‐1+ progenitor cell populations cultured on these nanotopographies. Nanotopographies affected the formation of adhesions on experimental substrates. Adhesion formation was prominent on planar control substrates and reduced on nanocrater and nan

Who reads Interactions with nanoscale topography: Adhesion quantification and signal transduction in cells of osteogenic and multipotent lineage?

Author

Manus J. P. Biggs; R. Geoff Richards; Nikolaj Gadegaard; Rebecca J. McMurray; Stanley Affrossman; Chris D. W. Wilkinson; Richard O. C. Oreffo; Mathew J. Dalby

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1549-3296)

Published

2008

Language

EN

Field

Engineering (Physical Sciences)