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Micro/nano-topography promotes osteogenic differentiation of bone marrow stem cells by regulating periostin expression 1 1 Introduction 1 2 Materials and methods 2 2.1 Nanotube preparation and morphological characterization 2 2.2 Measurements of contact angle and zeta potential 2 2.3 BMSCs isolation and culture 2 2.4 BMSCs flow cytometry identification assay 2 2.5 BMSCs osteogenic differentiation and adipogenic differentiation 2 2.6 Gene expression analysis 3 2.7 Immunofluorescent staining 3 2.8 Protein expression analyses 3 2.9 Postn siRNA 3 2.10 Alkaline phosphatase (ALP) activity assay and alizarin red S detection 3 2.11 Statistical analysis 4 3 Results 4 3.1 Surface characterization 4 3.2 Characteristics of BMSCs 4 3.3 Postn gene expression and its effect on osteogenic gene expression 4 3.3.1 Early effects 4 3.3.2 Later effects 4 3.4 Interaction between Postn and integrin 4 3.5 Role of Postn in phosphorylation of FAK and total FAK 4 3.6 The role of Postn for Wnt/β-catenin signaling pathway 7 4 Discussion 12 5 Conclusion 13 Ethics approval and consent to participate 13 Funding 14 Conflicts of interest 14 Data Availability 14 Appendix A Supporting information 14 References 14

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Language

EN

Category

nonfiction