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Establishment of a PPP2CA homozygous knockout human embryonic stem cell line via CRISPR/Cas9 system by Tongbin Ding; Zhe Gao; Hang Liu; Yuan Li; Shichao Liu; Liguo Jian is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Establishment of a PPP2CA homozygous knockout human embryonic stem cell line via CRISPR/Cas9 system about?

Protein phosphatase 2A (PPP2CA) is one of the four main Ser/Thr phosphatase enzymes, which involved in the negative control of cell growth and division. PPP2CA is the main protein phosphatase in the heart, which regulates Ca (2+) through a series of ion channels and transporters. In this study, we generated a PPP2CA homozygous knockout human embryonic stem cell line WAe009-A-25 based on the transient expression CRISPR/Cas9 system to investigate functional effect of PP1 deficiency. This cell line has multidirectional differentiation potential, normal karyotypic and trilineage differentiation potential in vivo.

Who reads Establishment of a PPP2CA homozygous knockout human embryonic stem cell line via CRISPR/Cas9 system?

Author

Tongbin Ding; Zhe Gao; Hang Liu; Yuan Li; Shichao Liu; Liguo Jian

Publisher

Elsevier BV

Published

2023

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)