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Effect of nickel(II) on DNA-protein binding, thymidine incorporation, and sedimentation pattern of chromatin fractions from intact mammalian cells by Steven R. Patierno; Masayasu Sugiyama; Max Costa is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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Nuclear uptake and chromatin binding of nickel(II) was investigated in Chinese hamster ovary (CHO) cells. The cytoplasmic:nuclear ratio of nickel immediately following treatment was 5:1, but by 24 and 48 hours this ratio decreased to 4:1 and 2:1, respectively, indicating that nickel is retained longer in the nucleus than cytoplasmic nickel. Chromatin was fractionated by sonication and centrifugation into fast-sedimenting, magnesium-insoluble, or magnesium-soluble components. The magnesium-insoluble portion bound more nickel ions and retained the metal longer than either the magnesium-soluble or the fast-sedimenting fractions. Treatment of cells with nickel chloride (NiCl2) decreased the amount of DNA in the magnesium-insoluble fraction but increased the amount of DNA in the fast-sedimenting chromatin fraction. The magnesium-insoluble fraction isolated from nickel-treated cells contained approximately ten times more [35-S]-methionine-labeled protein per milligram DNA compared with untreated cells. The magnesium-soluble and the fast-sedimenting fractions isolated from the nickel-treated cells did not exhibit a similar increase in [35-S]-methionine-labeled protein per milligram of DNA

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Author

Steven R. Patierno; Masayasu Sugiyama; Max Costa

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); VCH Publishers; Wiley (ISSN 0887-2082)

Published

1986

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)