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Can I read Microfluidics and Multielectrode Array-compatible Organotypic Slice Culture Method on EtoBox?
Microfluidics and Multielectrode Array-compatible Organotypic Slice Culture Method by Yevgeny Berdichevsky; Helen Sabolek; John B. Levine; Kevin J. Staley; Martin L. Yarmush is a Neuroscience article available to read on EtoBox.
What is Microfluidics and Multielectrode Array-compatible Organotypic Slice Culture Method about?
Organotypic brain slice cultures are used for a variety of molecular, electrophysiological, and imaging studies. However, the existing culture methods are difficult or expensive to apply in studies requiring long-term recordings with multielectrode arrays (MEAs). In this work, a novel method to maintain organotypic cultures of rodent hippocampus for several weeks on standard MEAs in an unmodified tissue culture incubator is described. Polydimethylsiloxane (Sylgard) mini-wells were used to stabilize organotypic cultures on glass and MEA surfaces. Hippocampus slices were successfully maintained within PDMS mini-wells for multiple weeks, with preserved pyramidal layer organization, connectivity, and activity. MEAs were used to record the development of spontaneous activity in an organotypic cultures for 4 weeks. This method is compatible with integration of microchannels into the culture substrate. Microchannels were incorporated into the mini-wells and applied to the guidance of axons originating within the slice, paving the way for studies of axonal sprouting using organotypic slices.
Who reads Microfluidics and Multielectrode Array-compatible Organotypic Slice Culture Method?
It is typically read by researchers, students, and practitioners in Neuroscience.
- Author
- Yevgeny Berdichevsky; Helen Sabolek; John B. Levine; Kevin J. Staley; Martin L. Yarmush
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0165-0270)
- Published
- 2009
- Language
- EN
- Field
- Neuroscience (Life Sciences)