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Validation of an in Vitro Model for Assessment of Androstenedione Hepatotoxicity Using the Rat Liver Cell Line Clone‐9 by Saura C. Sahu; Paddy L. Wiesenfeld; Chung S. Kim; Ivan A. Ross; Philip P. Sapienza; Richard Newell; Michael W. O'Donnell; Thomas J. Flynn is a Environmental Science article available to read on EtoBox.

What is Validation of an in Vitro Model for Assessment of Androstenedione Hepatotoxicity Using the Rat Liver Cell Line Clone‐9 about?

## Abstract Androstenedione, a naturally occurring steroid hormone, has been used to enhance athletic performance. Little is known, however, about its hepatotoxicity. Clone‐9 cells, a non‐transformed epithelial cell line that was originally isolated from normal liver of a 4‐week old Sprague‐Dawley rat, were used as an __in vitro__ model to assess the hepatotoxic potential of androstenedione. The cultures were treated with androstenedione for 24 h at 37 °C in 5% CO~2~ at concentrations of 0–100 μg ml^−1^. After the treatment period, the cells and the culture supernatants were assayed for markers of cytotoxicity which included: release of liver enzymes, cell viability, cellular double‐stranded DNA content, oxidative stress, steatosis, cellular ATP content, caspase‐3 activity, the mitochondrial permeability transition and induction of cytochrome P450 activity. Significant concentration‐dependent differences from control were observed in some endpoints at medium concentrations of 10 μg ml^−1^ and above. These __in vitro__ findings were compared with comparable endpoints obtained from an __in vivo__ study of androstenedione toxicity in female Sprague‐Dawley rats. Of the eight endpoints

Who reads Validation of an in Vitro Model for Assessment of Androstenedione Hepatotoxicity Using the Rat Liver Cell Line Clone‐9?

Author

Saura C. Sahu; Paddy L. Wiesenfeld; Chung S. Kim; Ivan A. Ross; Philip P. Sapienza; Richard Newell; Michael W. O'Donnell; Thomas J. Flynn

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0260-437X)

Published

2008

Language

EN

Field

Environmental Science (Physical Sciences)