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A Requirement for Cholesterol and Its Structural Features for a Human Macrophage-like Cell Line by Mojtaba Esfahani; Louis Scerbo; Thomas M. Devlin is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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The lipid requirements of a human macrophagelike cell line were studied. The cells grew only about one generation in a medium supplemented with delipidated serum; during the growth the cholesterol content of the cells was depleted. Growth was restored by including in the medium serum lipids subjected to alkaline hydrolysis or cholesterol. The extent of growth was dependent on cholesterol concentration. No growth was obtained with 5-cholestene, 5-cholesten-3-one, cholesteryl chloride, coprostanol, beta-sitosterol, or stigmasterol. Very limited growth occurred with cholesterol methylether, epicholesterol, or beta-cholestanol. Therefore, for optimal growth of these cells there is a stringent requirement for the structural features of cholesterol, which include a 3-beta OH group, a delta 5-double bond, a trans ring A/B configuration, and freedom of the side chain from bulky groups. This stringency far exceeds what was previously reported for other cells. Of the six sterols that failed to support growth at all, five were incorporated into cells moderately to extensively. This suggests that assembly of a functional membrane is impaired when these sterols are used as substrates for growth

Who reads A Requirement for Cholesterol and Its Structural Features for a Human Macrophage-like Cell Line?

Author

Mojtaba Esfahani; Louis Scerbo; Thomas M. Devlin

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)

Published

1983

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)