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Insect resistance to Nilaparvata lugens and Cnaphalocrocis medinalis in transgenic indica rice and the inheritance of gna + sbti transgenes by Guiying Li; Xinping Xu; Hengtai Xing; Huachen Zhu; Qin Fan is a Agricultural and Biological Sciences article available to read on EtoBox.

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## Abstract Molecular genetic analysis and insect bioassay of transgenic indica rice ‘Zhuxian B’ plants carrying snowdrop lectin gene (__gna__) and soybean trypsin inhibitor gene (__sbti__) were investigated in detail. PCR, ‘dot’ blot and PCR‐Southern blot analysis showed that both transgenes had been incorporated into the rice genome and transmitted up to R3 progeny in most lines tested. Some transgenic lines exhibited Mendelian segregation, but the other showed either 1:1 (positive: negative for the transgenes) or other aberrant segregation patterns. The segregation patterns of __gna__ gene crossed between R2 and R3 progeny. In half of transgenic R3 lines, __gna__ and __sbti__ transgenes co‐segregated. Two independent homozygous lines expressing double transgenes were identified in R3 progeny. Southern blot analysis demonstrated that the copy numbers of integrated __gna__ and __sbti__ transgenes varied from one to ten in different lines. Insect bioassay data showed that most transgenic plants had better resistance to both __Nilaparvata lugens__ (Ståhl) and __Cnaphalocrocis medinalis__ (Guenée) than wild‐type plants. The insect resistance of transgenic lines increased with the inc

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Author

Guiying Li; Xinping Xu; Hengtai Xing; Huachen Zhu; Qin Fan

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1526-498X)

Published

2004

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)