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Conformational analysis of the leukocyte-specific EF-hand protein p65/L-plastin by X-ray scattering in solution by Hiroto Shinomiya; Masaji Shinjo; Liu Fengzhi; Yoshihiro Asano; Hiroshi Kihara is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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p65/L-Plastin is a leukocyte-specific EF-hand protein which plays a vital role in organizing the actin cytoskeleton. Since its overall structural information has been largely unknown, we employed the X-ray scattering technique to elucidate the structure. Kratky plots of p65/L-plastin showed one peak, indicating that the protein takes compact globular conformations. The radii of gyration (Rg) of the monomer p65/L-plastin estimated from Guinier plots were 27.5 ± 0.5 Å and 28.6 Å in the absence and presence of Ca 2+ , respectively. The distance distribution function P(r) gave single peaks at 31.5-32.3 Å and 33 Å in the absence and presence of Ca 2+ , respectively. These indicate that p65/L-plastin becomes somewhat larger in the presence of Ca 2+ . The molecular shape of p65/L-plastin reconstructed from X-ray scattering data using the DAMMIN program has provided the first view of the overall structure of full-length plastin/fimbrin family proteins: a compact horseshoe-like shape with a small projection, which also exhibits Ca 2+ -induced conformational changes.

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Author

Hiroto Shinomiya; Masaji Shinjo; Liu Fengzhi; Yoshihiro Asano; Hiroshi Kihara

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0301-4622)

Published

2007

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)