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Sequence homology: A poor predictive value for profilins cross-reactivity by Mojtaba Sankian; Abdolreza Varasteh; Nazanin Pazouki; Mahmoud Mahmoudi is a Medicine article available to read on EtoBox.

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## Background Profilins are highly cross-reactive allergens which bind IgE antibodies of almost 20% of plant-allergic patients. This study is aimed at investigating cross-reactivity of melon profilin with other plant profilins and the role of the linear and conformational epitopes in human IgE cross-reactivity. ## Methods Seventeen patients with melon allergy were selected based on clinical history and a positive skin prick test to melon extract. Melon profilin has been cloned and expressed in __E. coli__. The IgE binding and cross-reactivity of the recombinant profilin were measured by ELISA and inhibition ELISA. The amino acid sequence of melon profilin was compared with other profilin sequences. A combination of chemical cleavage and immunoblotting techniques were used to define the role of conformational and linear epitopes in IgE binding. Comparative modeling was used to construct three-dimensional models of profilins and to assess theoretical impact of amino acid differences on conformational structure. ## Results Profilin was identified as a major IgE-binding component of melon. Alignment of amino acid sequences of melon profilin with other profilins showed the most identity

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Author

Mojtaba Sankian; Abdolreza Varasteh; Nazanin Pazouki; Mahmoud Mahmoudi

Publisher

BioMed Central; Springer (Biomed Central Ltd.); [London]: BioMed Central, c2003-; Springer Science and Business Media LLC (ISSN 1476-7961)

Published

2005

Language

EN

Field

Medicine (Health Sciences)