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Colorimetric detection of miRNA-21 by DNAzyme-coupled branched DNA constructs by Elyas Hosseinzadeh; Hadi Ravan; Abbas Mohammadi; Hossein Pourghadamyari is a Chemistry article available to read on EtoBox.

What is Colorimetric detection of miRNA-21 by DNAzyme-coupled branched DNA constructs about?

Fluctuation of nucleic acid expression and ultrasensitive and specific detection of these variations in expression is a crucial subject in molecular medicine and clinical theranostics. A novel DNAzyme-coupled branched hybridization chain reaction (b-HCR) assay is reported for efficient signal-amplified detection of miRNA in this study. This assay was composed of a translator (T) hybridized with miR-21 to initiate the first HCR by hairpin 1 (H) and hairpin 2 (H). The primary HCR provided a backbone chain for numerous branches budding through hairpin 3 (H) and hairpin 4 (H) assembles. In the presence of hemin, the G-rich domains embedded in H1 and H4 produce an active G-quadruplex DNAzyme upon exposure to a target that could catalyze the oxidation of colorless substrate to colored product. The present approach has the potential to be used for quantitative detection of miR-21 with a sensitivity and a dynamic range of 1 pM and 1 pM to 1 nM, respectively.

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Author

Elyas Hosseinzadeh; Hadi Ravan; Abbas Mohammadi; Hossein Pourghadamyari

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0039-9140)

Published

2020

Language

EN

Field

Chemistry (Physical Sciences)