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Enzyme activities and compositional shifts in the community structure of bacterial groups in English wetland soils associated with preservation of organic remains in archaeological sites by Isabel Douterelo; Raymond Goulder; Malcolm Lillie is a Environmental Science article available to read on EtoBox.

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To extend our previous research on preservation in situ of organic archaeological remains in wetland soils, physicochemical variables and extracellular enzyme activities (b-D-glucosidase, phosphatase, and leucine aminopeptidase) were measured in two archaeological wetland soils in northern England, from the soil surface down to 100 cm during two years. These two locations, with a proven potential to contain and preserve archaeo-environmental remains, were of interest because they had different hydrological and land-management history. In addition, for one of the sampling years, the structure of soil bacterial communities was monitored using polymerase chain reaction followed by denaturing gradient gel electrophoresis (PCR-DGGE) of 16S rRNA gene-coding fragments amplified from soil DNA, using primers specific for eu bacteria, actinomycetes, and a-proteobacteria. The physicochemical monitoring data indicated that at Sutton Common the buried conditions were less stable and more heterogeneous throughout the soil profile than at Hatfield Moor. Extracellular enzyme activities decreased with depth at both sites and higher average enzyme activity was found at Hatfield Moor than at Sutton C

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Author

Isabel Douterelo; Raymond Goulder; Malcolm Lillie

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0964-8305)

Published

2011

Language

EN

Field

Environmental Science (Physical Sciences)