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RUNX2 and ALP expression in osteoblast cells exposed by PMMA-HAp combination: An in vitro study by Claudia Michelle Darjanki; Chiquita Prahasanti; Eka Fitria A; Banun Kusumawardani; I Komang Evan Wijaksana; Mohammed Aljunaid is a Dentistry article available to read on EtoBox.

What is RUNX2 and ALP expression in osteoblast cells exposed by PMMA-HAp combination: An in vitro study about?

## Objective To observe the expression of Runt-Related Transcription Factors 2 (RUNX2) and Alkaline Phosphatase (ALP) markers in osteoblast cell cultures exposed to Polymethylmethacrylate (PMMA) combined with hydroxyapatite (HAp) material to improve osteointegration of bone implants. ## Methods Sample of PMMA and HAp materials with a mixture of PMMA with HAp made from limestone as natural source which processed through Balai Besar Keramik (HApBBK) in the first group and a mixture of PMMA with HAp made from bovine bone which processed through Good Manufacturing Practice (HApGMP) in the second group. Twenty-four fetal rat calvarie osteoblast cell cultures were randomly divided into 6 groups: 7- and 14-day control group, 7 and 14 days PMMA-HApGMP group, 7 and 14 days PMMA-HApBBK group. The expression of RUNX2 and ALP was seen by immunocytochemical examination. ## Result The one-way ANOVA with a significance value of 0.000 (p < 0.05). There was an increase in RUNX2 and ALP expressions on both PMMA-HApBBK and PMMA-HApGMP groups on days 7 and 14 in osteoblast cell cultures. ## Conclusion The PMMA-HApBBK and PMMA-HApGMP showed an increase in the RUNX2 and ALP expression in osteoblast cell

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Author

Claudia Michelle Darjanki; Chiquita Prahasanti; Eka Fitria A; Banun Kusumawardani; I Komang Evan Wijaksana; Mohammed Aljunaid

Publisher

Elsevier BV

Published

2023

Language

EN

Field

Dentistry (Health Sciences)