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Receptor-mediated Endocytosis of Trichosanthin in Choriocarcinoma Cells by Wood Yee Chan; Hai Huang; Siu-Cheung Tam is a Environmental Science article available to read on EtoBox.

What is Receptor-mediated Endocytosis of Trichosanthin in Choriocarcinoma Cells about?

Trichosanthin (TCS) is a ribosome inactivating protein (RIP). It is generally believed that its many biological activities act through inhibition of ribosomes resulting in a decrease in protein synthesis. It has been hypothesized that the rate of entry of TCS into cells to reach ribosomes is an important factor in determining its biological activity. To prove this hypothesis, we have mapped out and compared the intracellular routing of TCS in two cell lines, namely the choriocarcinoma JAR cell line, which is known to be highly sensitive to the toxic effects of TCS, and the hepatoma H35 cell line, to which TCS shows minimal toxicity. Results from laser scanning confocal microscopy indicated that fluorescein isothiocyanate labeled TCS quickly accumulated inside JAR cells within 4 h of incubation while only a low level of fluorescent signals was detected in H35 cells during the same period of time. When TCS was conjugated with gold particles (Au) and its intracellular locations were traced with a transmission electron microscope, it was found that most of TCS were bound to coated pits on the JAR cell surface and were rapidly internalized within an hour. By 4 h, TCS reached almost ever

Who reads Receptor-mediated Endocytosis of Trichosanthin in Choriocarcinoma Cells?

Author

Wood Yee Chan; Hai Huang; Siu-Cheung Tam

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0300-483X)

Published

2003

Language

EN

Field

Environmental Science (Life Sciences)