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Investigation of a plasmid containing a novel immunotoxin VEGF165-PE38 gene for antiangiogenic therapy in a malignant glioma model by Chang‐chen Hu; Hong‐ming Ji; Sheng‐li Chen; Han‐wei Zhang; Bin‐quan Wang; Li‐yuan Zhou; Zi‐ping Zhang; Xin‐lin Sun; Zhen‐zhou Chen; Ying‐qian Cai; Ling‐sha Qin; Li Lu; Xiao‐dan Jiang; Ru‐xiang Xu; Yi‐quan Ke is a Medicine article available to read on EtoBox.
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## Abstract Inhibition of tumor neovascularization has profound effects on the growth of solid tumors. Our previous studies have shown the effect of VEGF165‐PE38 recombinant immunotoxin on proliferation and apoptosis in human umbilical vein endothelial cells __in vitro__. In this study, we explored the direct inhibition of angiogenesis in chick chorioallantoic membrane and antiangiogenic therapy in a malignant glioma model. HEK293 cells were transfected with the pVEGF165PE38‐IRES2‐EGFP plasmid. ELISA was used to confirm the expression of VEGF165‐PE38 in the transfected cells. These cells released 1396 ± 131.9 pg VEGF165‐PE38/1×10^4^ cells/48 h into the culture medium and the supernatant was capable of inhibiting the growth of capillary‐like structures in chick chorioallantoic membrane assay. In a murine malignant glioma model, plasmid was directly administered via multiple local intratumoral delivery. After day 16 the tumor volume in mice treated with pVEGF165PE38‐IRES2‐EGFP was significantly lower than that in mice in the control groups. Immunohistochemistry studies showed that the treated group had decreased expression of CD31. Quantitative analysis of microvessel density in the
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- Author
- Chang‐chen Hu; Hong‐ming Ji; Sheng‐li Chen; Han‐wei Zhang; Bin‐quan Wang; Li‐yuan Zhou; Zi‐ping Zhang; Xin‐lin Sun; Zhen‐zhou Chen; Ying‐qian Cai; Ling‐sha Qin; Li Lu; Xiao‐dan Jiang; Ru‐xiang Xu; Yi‐quan Ke
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)
- Published
- 2010
- Language
- EN
- Field
- Medicine (Health Sciences)