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Induction of cell cycle arrest and apoptosis in human prostate carcinoma cells by a recombinant adenovirus expressing p27(Kip1) by Adrienne L. Katner; Quoc Bao Ly Hoang; Praveen Gootam; Ewa Jaruga; Quangwei Ma; James Gnarra; Walter Rayford is a Medicine article available to read on EtoBox.

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## Background: Adp27(kip1), a recombinant adenovirus, was evaluated for expression of p27, a cyclin-dependent kinase inhibitor (cdki) and tumor suppressor protein, in human prostate carcinoma cells. effects of p27(kip1) on cell cycle and apoptosis were analyzed. ## Methods: We evaluated the effects of overexpression of p27(kip1) in the human prostate carcinoma cell lines lncap, du-145, and pc-3 in vitro and in vivo. growth curve studies, cell cycle analysis, terminal deoxynucleotidyl transferase-mediated nick end-labeling (tunel), and annexin v-fluorescein isothiocyanate apoptosis analyses were conducted to determine effects of p27(kip1) on cell cycle. cdki activity assays and western blots were conducted to determine presence/activities of cdkis. ## Results: Adp27(kip1)-induced protein levels increased in a dose-dependent manner; p27(kip1) protein was detected within 6 hr of infection with adp27(kip1) and remained stable for at least 48 hr. the activities of cdk2, cdk4, and cdc2 kinases were inhibited 24 hr after infection with adp27(kip1). bromodeoxyuridine incorporation demonstrated a dose-dependent decrease in s-phase cells 24 hr postinfection. tunel analysis revealed an induct

Who reads Induction of cell cycle arrest and apoptosis in human prostate carcinoma cells by a recombinant adenovirus expressing p27(Kip1)?

Author

Adrienne L. Katner; Quoc Bao Ly Hoang; Praveen Gootam; Ewa Jaruga; Quangwei Ma; James Gnarra; Walter Rayford

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0270-4137)

Published

2002

Language

EN

Field

Medicine (Health Sciences)