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Biochemical Composition of the Superficial Layer of Articular Cartilage by R. Crockett; A. Grubelnik; S. Roos; C. Dora; W. Born; H. Troxler is a Engineering article available to read on EtoBox.

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## Abstract To gain more information on the mechanism of lubrication in articular joints, the superficial layer of bovine articular cartilage was mechanically removed in a sheet of ice that formed on freezing the cartilage. Freeze‐dried samples contained low concentrations of chondroitin sulphate and protein. Analysis of the protein by SDS PAGE showed that the composition of the sample was comparable to that of synovial fluid (SF). Attenuated total reflection infrared (ATR‐IR) spectroscopy of the dried residue indicated that the sample contained mostly hyaluronan. Moreover, ATR‐IR spectroscopy of the upper layer of the superficial layer, adsorbed onto silicon, showed the presence of phospholipids. A gel could be formed by mixing hyaluronan and phosphatidylcholine in water with mechanical properties similar to those of the superficial layer on cartilage. Much like the superficial layer of natural cartilage, the surface of this gel became hydrophobic on drying out. Thus, it is proposed that the superficial layer forms from hyaluronan and phospholipids, which associate by hydrophobic interactions between the alkyl chains of the phospholipids and the hydrophobic faces of the disacchari

Who reads Biochemical Composition of the Superficial Layer of Articular Cartilage?

Author

R. Crockett; A. Grubelnik; S. Roos; C. Dora; W. Born; H. Troxler

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1549-3296)

Published

2007

Language

EN

Field

Engineering (Physical Sciences)