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Altered Gene Expression in Acute Systemic Inflammation Detected by Complete Coverage of the Human Liver Transcriptome by Cédric Coulouarn; Grégory Lefebvre; Céline Derambure; Thierry Lequerre; Michel Scotte; Arnaud Francois; Dominique Cellier; Maryvonne Daveau; Jean-Philippe Salier is a Medicine article available to read on EtoBox.
What is Altered Gene Expression in Acute Systemic Inflammation Detected by Complete Coverage of the Human Liver Transcriptome about?
The goal of the current study was to provide complete coverage of the liver transcriptome with human probes corresponding to every gene expressed in embryonic, adult, and/or cancerous liver. We developed dedicated tools, namely, the Liverpool nylon array of complementary DNA (cDNA) probes for approximately 10,000 nonredundant genes and the LiverTools database. Inflammation-induced transcriptome changes were studied in liver tissue samples from patients with an acute systemic inflammation and from control subjects. One hundred and fifty-four messenger RNAs (mRNA) correlated statistically with the extent of inflammation. Of these, 134 mRNA samples were not associated previously with an acute-phase (AP) response. The hepatocyte origin and proinflammatory cytokine responsiveness of these mRNAs were confirmed by quantitative reverse-transcription polymerase chain reaction (Q-RT-PCR) in cytokine-challenged hepatoma cells. The corresponding gene promoters were enriched in potential binding sites for inflammation-driven transcription factors in the liver. Some of the corresponding proteins may provide novel blood markers of clinical relevance. The mRNAs whose level is most correlated with
Who reads Altered Gene Expression in Acute Systemic Inflammation Detected by Complete Coverage of the Human Liver Transcriptome?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Cédric Coulouarn; Grégory Lefebvre; Céline Derambure; Thierry Lequerre; Michel Scotte; Arnaud Francois; Dominique Cellier; Maryvonne Daveau; Jean-Philippe Salier
- Publisher
- Ovid Technologies (Wolters Kluwer Health)
- Published
- 2004
- Language
- EN
- Field
- Medicine (Health Sciences)