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Integrating Human Stem Cell Expansion and Neuronal Differentiation in Bioreactors by Margarida Serra; Catarina Brito; Eunice M Costa; Marcos FQ Sousa; Paula M Alves is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract ## Background Human stem cells are cellular resources with outstanding potential for cell therapy. However, for the fulfillment of this application, major challenges remain to be met. Of paramount importance is the development of robust systems for __in vitro__ stem cell expansion and differentiation. In this work, we successfully developed an efficient scalable bioprocess for the fast production of human neurons. ## Results The expansion of undifferentiated human embryonal carcinoma stem cells (NTera2/cl.D1 cell line) as 3D-aggregates was firstly optimized in spinner vessel. The media exchange operation mode with an inoculum concentration of 4 × 10^5^ cell/mL was the most efficient strategy tested, with a 4.6-fold increase in cell concentration achieved in 5 days. These results were validated in a bioreactor where similar profile and metabolic performance were obtained. Furthermore, characterization of the expanded population by immunofluorescence microscopy and flow cytometry showed that NT2 cells maintained their stem cell characteristics along the bioreactor culture time. Finally, the neuronal differentiation step was integrated in the bioreactor process, by additio

Who reads Integrating Human Stem Cell Expansion and Neuronal Differentiation in Bioreactors?

Author

Margarida Serra; Catarina Brito; Eunice M Costa; Marcos FQ Sousa; Paula M Alves

Publisher

BioMed Central; Springer (Biomed Central Ltd.); [London]: BioMed Central, 2001-; Springer Science and Business Media LLC (ISSN 1472-6750)

Published

2009

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)