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Expression of carbonic anhydrase IX in human pancreatic cancer by M. Juhász; J. Chen; U. Lendeckel; U. Kellner; H.-U. Kasper; Z. Tulassay; S. Pastorekova; P. Malfertheiner; M. P. A. Ebert is a Medicine article available to read on EtoBox.

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## Summary **Background** : Carbonic anhydrase IX has been linked to cancer development and progression. **Aim** : To analyse carbonic anhydrase IX expression and anhydrase inhibition in pancreatic cancer and to correlate these findings with p53 expression and microvessel density. **Materials and methods** : Seventy‐seven pancreatic cancers were examined (43 males, 34 females; mean age, 64 years). The anti‐carbonic anhydrase IX M75 antibody was used for immunohistochemistry and Western blot analysis. Microvessels were visualized using the anti‐CD34 antibody, and p53 expression in cancer cells was assessed with a specific anti‐p53 antibody. Quantitative polymerase chain reaction was performed in order to assess carbonic anhydrase IX mRNA levels in the pancreas. Furthermore, pancreatic cancer cell lines were treated with acetazolamide, a carbonic anhydrase inhibitor. **Results** : In the normal pancreas, carbonic anhydrase IX immunoreactivity was observed at the basolateral membrane of ductal cells in 24 cases (31%). Carbonic anhydrase IX expression was found at the membrane and in the cytoplasm of pancreatic cancer cells in 16 pancreatic cancers (21%). Carbonic anhydrase IX expressi

Who reads Expression of carbonic anhydrase IX in human pancreatic cancer?

Author

M. Juhász; J. Chen; U. Lendeckel; U. Kellner; H.-U. Kasper; Z. Tulassay; S. Pastorekova; P. Malfertheiner; M. P. A. Ebert

Publisher

John Wiley and Sons; Blackwell Publishing; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley; Research Square (ISSN 0269-2813)

Published

2003

Language

EN

Field

Medicine (Health Sciences)