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Molecular cloning, characterization, and expression analysis of lipopolysaccharide-induced TNF-α factor (LITAF) in the mud crab, Scylla paramamosain by Yue Wu; Yuxiao Sun; Wanrong Zhang; Jingjing Yang; Xiefei Zhou; Liaoyi Xu; Peichen Wang; Haisheng Xu is a Environmental Science article available to read on EtoBox.
What is Molecular cloning, characterization, and expression analysis of lipopolysaccharide-induced TNF-α factor (LITAF) in the mud crab, Scylla paramamosain about?
## Abstract Lipopolysaccharide-induced TNF-α factor (LITAF) is an important transcription factor mediating the expression of inflammatory cytokines, including TNF-α, which is involved in the innate immune defence against infectious agents. In this study, the Scylla paramamosain LITAF (LITAF) gene was cloned and identified from S. paramamosain using RT-PCR and RACE. The full length cDNA of LITAF was 1116 bp, contained an ORF of 495 bp encoding a polypeptide of 164 amino acids with predicted molecular mass of 17.86 kDa and a theoretical isoelectric point of 8.51. Sequence alignment showed that LITAF possessed a typical LITAF domain with two CXXC motifs and eight conserved cysteine residues. Phylogenetic analysis indicated that LITAF clustered closely with known LITAFs from Eriocheir sinensis and Litopenaeus vannamei. qRT-PCR analysis showed LITAF mRNA transcript expressed ubiquitously in all examined tissues with the highest expression in haemocytes, while lower expression in hepatopancreas. Its expression levels displayed a trend of up-regulation and then returned to its initial value in haemocytes and gill, post challenge with LPS, Poly (I : C), and Vibrio alginolyticus, respective
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It is typically read by researchers, students, and practitioners in Environmental Science.
- Author
- Yue Wu; Yuxiao Sun; Wanrong Zhang; Jingjing Yang; Xiefei Zhou; Liaoyi Xu; Peichen Wang; Haisheng Xu
- Publisher
- Brill (ISSN 0011-216X)
- Published
- 2020
- Language
- EN
- Field
- Environmental Science (Physical Sciences)