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Purification and cDNA cloning of nitric oxide reductase cytochrome P450nor (CYP55A4) from Trichosporon cutaneum by Li Zhang; Naoki Takaya; Tatsuya Kitazume; Toshihiro Kondo; Hirofumi Shoun is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Purification and cDNA cloning of nitric oxide reductase cytochrome P450nor (CYP55A4) from Trichosporon cutaneum about?

Cytochrome P450nor is involved in fungal denitrification as nitric oxide (NO) reductase. Although the heme protein has been known to occur in restricted species of fungi that belong to ascomycotina, we have previously suggested that it would also occur in the yeast __Trichosporon cutaneum__, which is phylogenetically far from those P450nor‐producing ascomycetous fungi. Here we isolated and characterized the heme protein from the basidiomycetous yeast __T__. __cutaneum__. P450nor of the yeast (TcP450nor) exhibited properties in terms of catalysis, absorption spectrum and molecular mass that are almost identical to those of its counterparts in ascomycetous fungi. We also isolated and sequenced its cDNA. The predicted primary structure of TcP450nor showed high sequence identities (around 65%) to those of other P450nors, indicating that they belong to the same family. TcP450nor protein cofractionated with cytochrome __c__ oxidase by subcellular fractionation and its predicted primary structure contained an extension on its amino terminus that is characteristic of a mitochondrial‐targeting signal, indicating that it is a mitochondrial protein like some of the isoforms of other fungi. On

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Author

Li Zhang; Naoki Takaya; Tatsuya Kitazume; Toshihiro Kondo; Hirofumi Shoun

Publisher

John Wiley and Sons; Springer; Wiley (Blackwell Publishing); Springer-Verlag; Blackwell Publishing Inc.; Springer Verlag; Wiley; Springer Science and Business Media LLC (ISSN 1432-1327)

Published

2001

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)