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Production of doubled haploid plants in cucumber (Cucumis sativus L.) via parthenogenesis by Hamed Salehian; Sahar Shahnazi; Mohamadreza Nazari is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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Double haploid production methods have great potential for accelerating breeding programs for cultivar development. Parthenogenesis, an embryo arising from unfertilized egg cells, is the most efficient technique for Cucumis sativus L. breeding programs. This study focused on the creation of haploid plants in cucumbers through the fertilization of female flowers with gamma radiation-treated pollen. The anthers were treated with different doses of gamma rays (200.0, 300.0, and 500.0 Gray). Pollen viability and vigor were investigated using the TTC (2,3,5-triphenyl tetrazolium chloride) and germination tests, respectively. Isolated female flowers were pollinated with irradiation-affected pollen. According to the results, pollination is most effective in producing haploid offspring when pollen has been exposed to radiation at a dose of 300.0 Gy. The ploidy levels of the regenerated plantlets were determined and confirmed using flow cytometry. In most plants, 78.39% were found to have only half the normal number of chromosomes (haploid), and 21.6% had a mix of normal and half chromosomes (mixoploid). The number of chromosomes in haploid plants was doubled by in vitro treatment of nodal

Who reads Production of doubled haploid plants in cucumber (Cucumis sativus L.) via parthenogenesis?

Author

Hamed Salehian; Sahar Shahnazi; Mohamadreza Nazari

Publisher

Springer Science and Business Media LLC

Published

2023

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)