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What is Understanding Fluorescence Spectroscopy about?
Fluorescence spectroscopy is a type of electromagnetic spectroscopy that analyzes fluorescence from a sample using ultraviolet light, which excites electrons in certain compounds causing them to emit light of lower energy. It involves three phases: fluorescence, vibrational relaxation, and phosphorescence. The ratio of photons emitted to photons absorbed is known as quantum efficiency or quantum yield, denoted by ф. Factors that affect fluorescence include concentration, quantum yield, intensity of incident
- Author
- Wajeeha Mehak
- Language
- EN