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Rapid Screening and Confirmation of Drugs and Toxic Compounds in Biological Specimens Using Liquid Chromatography/ion Trap Tandem Mass Spectrometry and Automated Library Search by Hsiu-Chuan Liu; Ray H. Liu; Dong-Liang Lin; Hsiu-O Ho is a Chemistry article available to read on EtoBox.
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## Abstract Recent advances in liquid chromatography/tandem mass spectrometry (LC/MS/MS) technology have provided an opportunity for the development of more specific approaches to achieve the ‘screen’ and ‘confirmation’ goals in a single analytical step. For this purpose, this study adapts the electrospray ionization ion trap LC/MS/MS instrumentation (LC/ESI‐MS/MS) for the screening and confirmation of over 800 drugs and toxic compounds in biological specimens. Liquid‐liquid and solid‐phase extraction protocols were coupled to LC/ESI‐MS/MS using a 1.8‐μm particle size analytical column operated at 50°C. Gradient elution of the analytes was conducted using a solvent system composed of methanol and water containing 0.1% formic acid. Positive‐ion ESI‐MS/MS spectra and retention times for each of the 800 drugs and toxic compounds were first established using 1–10 μg/mL standard solutions. This spectra and retention time information was then transferred to the library and searched by the identification algorithm for the confirmation of compounds found in test specimens – based on retention time matches and scores of fit, reverse fit, and purity resulting from the searching process. The
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- Author
- Hsiu-Chuan Liu; Ray H. Liu; Dong-Liang Lin; Hsiu-O Ho
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0951-4198)
- Published
- 2009
- Language
- EN
- Field
- Chemistry (Physical Sciences)