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Enzymatically stable 5′ mRNA cap analogs: Synthesis and binding studies with human DcpS decapping enzyme by Marcin Kalek; Jacek Jemielity; Zbigniew M. Darzynkiewicz; Elzbieta Bojarska; Janusz Stepinski; Ryszard Stolarski; Richard E. Davis; Edward Darzynkiewicz is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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Four novel 5 0 mRNA cap analogs have been synthesized with one of the pyrophosphate bridge oxygen atoms of the triphosphate linkage replaced with a methylene group. The analogs were prepared via reaction of nucleoside phosphor/phosphon-1-imidazolidates with nucleoside phosphate/phosphonate in the presence of ZnCl 2 . Three of the new cap analogs are completely resistant to degradation by human DcpS, the enzyme responsible for hydrolysis of free cap resulting from 3 0 to 5 0 cellular mRNA decay. One of the new analogs has very high affinity for binding to human DcpS. Two of these analogs are Anti Reverse Cap Analogs which ensures that they are incorporated into mRNA chains exclusively in the correct orientation. These new cap analogs should be useful in a variety of biochemical studies, in the analysis of the cellular function of decapping enzymes, and as a basis for further development of modified cap analogs as potential anti-cancer and anti-parasite drugs.

Who reads Enzymatically stable 5′ mRNA cap analogs: Synthesis and binding studies with human DcpS decapping enzyme?

Author

Marcin Kalek; Jacek Jemielity; Zbigniew M. Darzynkiewicz; Elzbieta Bojarska; Janusz Stepinski; Ryszard Stolarski; Richard E. Davis; Edward Darzynkiewicz

Publisher

Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0968-0896)

Published

2006

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)