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A Model of Experimental Kidney Irradiation for Screening of Response Modifiers: Evaluation of Insulin-like Growth Factor-1 (IGF-1) and Erythropoietin (EPO) by A. Schnaiter; L. Cai; N.H. Andratschke; S. Schill; W.A. Weber; M. Molls; C. Nieder is a Medicine article available to read on EtoBox.
What is A Model of Experimental Kidney Irradiation for Screening of Response Modifiers: Evaluation of Insulin-like Growth Factor-1 (IGF-1) and Erythropoietin (EPO) about?
Materials/Methods: C57BL/6NHsd mice were irradiated to 20 Gy to the pulmonary cavity. Bone marrow stromal cell lines were isolated from Smad3ϩ/ϩ or Smad3Ϫ/Ϫ mice and transfected with a retrovirus containing DS-red or GFP transgene, respectively. At 80 days after irradiation, subgroups of mice were injected intravenously (IV) with cells from the DS-red-Smad3ϩ/ϩ or GFP-Smad3Ϫ/Ϫ bone marrow stromal cell line. In other subgroups, mice were injected intraperitoneally (IP) for 15 days (Monday-Friday for 3 weeks) with monoclonal rat anti-mouse antibodies to either T-cells (T1B-207) or macrophages (M1/70). All mice were followed for the development of irradiation-induced fibrosis. Results: In areas of organizing alveolitis/fibrosis, we detected both GFP Smad3Ϫ/Ϫ cells and DS-red Smad3ϩ/ϩ cells. However, there was increased accumulation of Smad3ϩ/ϩ DS-red cells compared to GFP Smad3Ϫ/Ϫ cells. In mice treated with anti-macrophage antibody, M1/70 antibody treated animals had increased survival compared to mice that received irradiation only (p ϭ 0.0225) or T1B-207 antibody. Conclusions: Migration of both marrow origin macrophages and fibroblasts play a critical role in the development of pulm
Who reads A Model of Experimental Kidney Irradiation for Screening of Response Modifiers: Evaluation of Insulin-like Growth Factor-1 (IGF-1) and Erythropoietin (EPO)?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- A. Schnaiter; L. Cai; N.H. Andratschke; S. Schill; W.A. Weber; M. Molls; C. Nieder
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0360-3016)
- Published
- 2005
- Language
- EN
- Field
- Medicine (Health Sciences)