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Characterization of proliferating human skeletal muscle-derived cells in vitro: Differential modulation of myoblast markers by TGF-β2 by Jeffrey D. Stewart; Terése L. Masi; Andrew E. Cumming; Gyongyi M. Molnar; Bruce M. Wentworth; Kuber Sampath; John M. McPherson; Peter C. Yaeger is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
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## Abstract Adult human skeletal muscle‐derived cells (HuSkMC) propagated in vitro are under investigation as a cell‐based therapy for the treatment of myocardial infarction. We have characterized HuSkMC with respect to cell identity and state of differentiation as a prerequisite to their clinical use. Flow cytometric analysis of propagated HuSkMC revealed a population of cells that expressed the myoblast markers CD56 and desmin. The presence of myoblasts in these cultures was further confirmed by their capacity to form myotubes and increase creatine kinase activity when cultured in low serum conditions. The non‐myoblast fraction of these propagated cells expressed TE7, a marker associated with the fibroblast phenotype. Spontaneous differentiation of myoblasts occurred during serial propagation of HuSkMC, as judged by myotube formation, thereby reducing the myoblast representative fraction with continued cell expansion. We examined transforming growth factor β2 (TGF‐β2) for its utility in controlling this spontaneous differentiation of adult human myoblasts in vitro. Propagation of HuSkMC in the presence of 1 ng/ml TGF‐β2 for 5 days decreased desmin expression within the myoblast p
Who reads Characterization of proliferating human skeletal muscle-derived cells in vitro: Differential modulation of myoblast markers by TGF-β2?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Jeffrey D. Stewart; Terése L. Masi; Andrew E. Cumming; Gyongyi M. Molnar; Bruce M. Wentworth; Kuber Sampath; John M. McPherson; Peter C. Yaeger
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)
- Published
- 2003
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)