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NLRP3 inflammasome is expressed and regulated in human islets by Lebreton, Fanny; Berishvili, Ekaterine; Parnaud, Géraldine; Rouget, Caroline; Bosco, Domenico; Berney, Thierry; Lavallard, Vanessa is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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NRLP3 inflammasome is a protein complex involved in the maturation of IL1β. In the onset of type 1 diabetes as well as in islet transplantation, IL-1β is one of the cytokines involved in the recruitment of immune cells in islets and eventually in islet destruction. Whether IL-1β is produced by islet cells is still under debate and NLRP3 inflammasome-dependent IL-1β production has not been yet determined in human islets. The aim of this study was to determine the expression and the regulation of the NRLP3 inflammasome in human islets. Human islets were stimulated with LPS and successively with ATP (LPS + ATP) in the presence or absence of the inflammasome inhibitor glyburide. Islets were also incubated in hypoxic or normoxic conditions for 24 h in the presence or absence of glyburide. Then, __IL1B__ and __NLRP3__ expression was studied by real time PCR, protein expression by western blot, protein localization by immunofluorescence and protein secretion by ELISA. LPS + ATP increased gene expression of __NRLP3__ and __IL1B__. Glyburide partially prevented this effect. IL-1β protein was localized in β and non-β cells. Moreover, LPS + ATP increased IL-1β protein expression and productio

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Author

Lebreton, Fanny; Berishvili, Ekaterine; Parnaud, Géraldine; Rouget, Caroline; Bosco, Domenico; Berney, Thierry; Lavallard, Vanessa

Publisher

Nature Publishing Group; Springer Science and Business Media LLC; London: Nature Publishing Group, 2001- (ISSN 2041-4889)

Published

2018

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)