About this Medicine article

Epithelial to mesenchymal transition in head and neck squamous carcinoma : Association of Src activation with E-cadherin down-regulation, vimentin expression, and aggressive tumor features by Mahitosh Mandal; Jeffery N. Myers; Scott M. Lippman; Faye M. Johnson; Michelle D. Williams; Suresh Rayala; Kazufumi Ohshiro; David I. Rosenthal; Randal S. Weber; Gary E. Gallick; Adel K. El-Naggar is a Medicine article available to read on EtoBox.

## Abstract ## BACKGROUND Epithelial–mesenchymal transformations (EMT) are critical for the invasion, progression, and metastasis of epithelial carcinogenesis. The role of EMT in head and neck squamous carcinoma (HNSC) tumorigenesis remains unexplored. In the current study, the expressions of several factors associated with the induction of EMT in HNSC cell lines and tumor specimens were investigated to define their functional and pathologic role in HNSC. ## METHODS Eleven HNSC cell lines and 50 primary tumor tissue specimens formed the materials of this study. Western blot analysis as well as immunohistochemical, and functional techniques were used to assess the status of activated Src (p‐Src), E‐cadherin, and vimentin in both cell lines and tumor tissues and the results were correlated with patients' clinicopathologic parameters. ## RESULTS The results demonstrated the inverse expression of p‐Src and E‐cadherin in the majority of cell lines and in primary tumor tissues compared with normal squamous mucosa. Elevated levels of p‐Src were accompanied by down‐regulation of E‐cadherin and the expression of vimentin in epithelial tumor cells. In vitro inhibition of Src led to E‐cadheri

Author

Mahitosh Mandal; Jeffery N. Myers; Scott M. Lippman; Faye M. Johnson; Michelle D. Williams; Suresh Rayala; Kazufumi Ohshiro; David I. Rosenthal; Randal S. Weber; Gary E. Gallick; Adel K. El-Naggar

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0008-543X)

Published

2008

Language

EN

Field

Medicine (Health Sciences)