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This document describes the chromatographic conditions and sample preparation method for analyzing drugs in plasma samples using HPLC. A C12/C8/C18 column with methanol, acetonitrile, and heptanesulfonic acid buffer as the mobile phase was used. Calibration standards of various concentrations of the drugs PZA, INH, and RFP along with an internal standard were prepared. Plasma samples were processed by adding internal standard and centrifuging to extract the drugs. The method was validated by assessing linea

Author

bkadlakha

Language

EN